Coding

Part:BBa_K1315002:Design

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-25)


The rpfC gene from Xanthomonas campestris


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 410
    Illegal NgoMIV site found at 505
    Illegal NgoMIV site found at 1332
    Illegal NgoMIV site found at 1423
    Illegal NgoMIV site found at 1938
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had 1 EcoRI site and 3 PstI sites that were all mutated out by site-directed mutagenesis.


Source

The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.

References